Biophysics of Molecular Chaperones: Function, Mechanisms and Client Protein Interactions
Molecular chaperones are critical to control protein quality in all living cells. Understanding chaperone function at the atomic level, and in particular its mode of interaction with client proteins, is crucial to understanding the fundamental roles chaperones play in biology. This book fills a gap in the literature by comprehensively summarizing and discussing new advanced experimental techniques for their analysis. Providing a comprehensive overview of advanced biophysical methods for the characterization of molecular mechanisms of molecular chaperones, the majority of the contributions are NMR methodology. This is the method of choice for atomic resolution studies of such systems. Additional notable biophysical approaches are considered to present all relevant current developments in exploring chaperone function and the transient and dynamic interactions with their client proteins.
The book is targeted at both current practitioners of structural biology and biophysical chemistry and scientists who are interested in entering the field. It could be useful for graduate students as supplementary reading.
Biophysics of Molecular Chaperones: Function, Mechanisms and Client Protein Interactions, Royal Society of Chemistry, 2023.
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Introduction: Molecular Chaperones and Protein Quality Controlp1-37ByD. Balchin;D. BalchinaProtein Biogenesis Laboratory, The Francis Crick Institute, London, UKSearch for other works by this author on:M. Aguilar Rangel;M. Aguilar RangelbDepartment of Structural Biology, Stanford University, Stanford, California, USASearch for other works by this author on:R. S. SamantR. S. SamantcSignalling Programme, The Babraham Institute, Cambridge, UKSearch for other works by this author on:
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Chapter 2: Structural Disorder in Chaperone Functions Probed by NMRp38-54ByY. Hu;Y. HuaKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, ChinabUniversity of Chinese Academy of Sciences, Beijing, 10049, ChinaSearch for other works by this author on:M. LiuM. LiuaKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, ChinabUniversity of Chinese Academy of Sciences, Beijing, 10049, ChinaSearch for other works by this author on:
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Chapter 3: Solution NMR Approaches for Studying Molecular Chaperonesp55-85ByE. E. Selig;E. E. SeligGreehey Children’s Cancer Research Institute and Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USASearch for other works by this author on:D. S. LibichD. S. LibichGreehey Children’s Cancer Research Institute and Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USASearch for other works by this author on:
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Chapter 4: Solution NMR Studies of Chaperone–Client Systemsp86-135ByC. Wan;C. WanMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular Dynamics, School of Life Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, ChinaSearch for other works by this author on:C. HuangC. HuangMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular Dynamics, School of Life Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, ChinaSearch for other works by this author on:
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Chapter 5: Preparing Chaperone–Client Protein Complexes for Biophysical and Structural Studiesp136-161ByI. Sučec;I. SučecaUniv. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale (IBS), 71, avenue des martyrs, F-38044 Grenoble, FranceSearch for other works by this author on:P. SchandaP. SchandabInstitute of Science and Technology Austria, Am Campus 1, A-3400 Klosterneuburg, AustriaSearch for other works by this author on:
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Chapter 6: NMR Study of the Structure and Dynamics of Chaperone–Client Complexesp162-180ByT. SaioT. SaioaDivision of Molecular Life Science, Institute of Advanced Medical Sciences, Tokushima University, Tokushima 770-8503, Tokushima, JapanbFujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, Tokushima University, Tokushima 770-8503, Tokushima, JapanSearch for other works by this author on:
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Chapter 7: Single Molecule Fluorescence Methods for Molecular Chaperones and Their Client Interactionsp181-198ByT. Mondol;T. MondolInstitute of Physical Chemistry and Signalling Research Centers BIOSS and CIBSS, University of Freiburg, GermanySearch for other works by this author on:B. Sohmen;B. SohmenInstitute of Physical Chemistry and Signalling Research Centers BIOSS and CIBSS, University of Freiburg, GermanySearch for other works by this author on:T. HugelT. HugelInstitute of Physical Chemistry and Signalling Research Centers BIOSS and CIBSS, University of Freiburg, GermanySearch for other works by this author on:
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Chapter 8: Visualization of Chaperone Mediated Protein Folding Using X-ray Crystallographyp199-216ByK. Wu;K. WuaDepartment of Molecular, Cellular, and Developmental Biology and Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI, USASearch for other works by this author on:S. Horowitz;S. HorowitzbDepartment of Chemistry & Biochemistry, Knoebel Institute for Healthy Aging, University of Denver, Denver, CO, USASearch for other works by this author on:J. C. A. BardwellJ. C. A. BardwellaDepartment of Molecular, Cellular, and Developmental Biology and Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI, USASearch for other works by this author on:
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Chapter 9: Studying Molecular Chaperones and Their Client Interactions by Nanometer Distance Restraints from Electron Paramagnetic Resonance Spectroscopyp217-241ByJ. Dröden;J. DrödenDepartment of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, GermanySearch for other works by this author on:M. DrescherM. DrescherDepartment of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, GermanySearch for other works by this author on:
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Chapter 10: EPR Studies of Chaperone Interactions and Dynamicsp242-277ByA. Siegel;A. SiegelaDivision of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd, CA 91125, USASearch for other works by this author on:J. Singh;J. SinghbDepartment of Chemistry, University of Southern California, 3430 S. Vermont Ave, CA 90089, USASearch for other works by this author on:P. Z. Qin;P. Z. QinbDepartment of Chemistry, University of Southern California, 3430 S. Vermont Ave, CA 90089, USAcDepartment of Biological Sciences, University of Southern California, 3430 S. Vermont Ave, CA 90089, USASearch for other works by this author on:S. O. ShanS. O. ShanaDivision of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd, CA 91125, USASearch for other works by this author on:
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Chapter 11: Probing Single Chaperone Substratesp278-318ByF. Wruck;F. WruckaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandsbLUMICKS BV, Amsterdam, The NetherlandsSearch for other works by this author on:M. J. Avellaneda;M. J. AvellanedaaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandscInstitute of Science and Technology Austria, Am Campus 1, Klosterneuburg 3400, AustriaSearch for other works by this author on:M. M. Naqvi;M. M. NaqviaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandsdDepartment of Pharmacology, University of Cambridge, CB2 1PD Cambridge, UKSearch for other works by this author on:E. J. Koers;E. J. KoersaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandseCentre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UKSearch for other works by this author on:K. Till;K. TillaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandsSearch for other works by this author on:L. Gross;L. GrossaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandsSearch for other works by this author on:F. Moayed;F. MoayedaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandsSearch for other works by this author on:A. Roland;A. RolandaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandsSearch for other works by this author on:L. W. H. J. Heling;L. W. H. J. HelingfMedical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Science, Leiden University, 2333CC, Leiden, The NetherlandsgCentre for Interdisciplinary Genome Research, Faculty of Science, Leiden University, Einsteinweg 55, 2333CC Leiden, The NetherlandsSearch for other works by this author on:A. Mashaghi;A. MashaghifMedical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Science, Leiden University, 2333CC, Leiden, The NetherlandsgCentre for Interdisciplinary Genome Research, Faculty of Science, Leiden University, Einsteinweg 55, 2333CC Leiden, The NetherlandsSearch for other works by this author on:S. J. TansS. J. TansaAMOLF, Science Park 104, 1098 XG Amsterdam, The NetherlandshBionanoscience Department of Delft University of Technology and Kavli Institute of Nanoscience Delft, 2629HZ Delft, The NetherlandsSearch for other works by this author on:
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Chapter 12: Integrative Methods to Investigate Chaperones in Regulating Protein Phase Separation and Aggregationp319-342ByD. Li;D. LiaBio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200030, ChinabZhangjiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai 200240, ChinaSearch for other works by this author on:S. Zhang;S. ZhangcInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, ChinaSearch for other works by this author on:C. LiuC. LiucInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, ChinaSearch for other works by this author on:
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Chapter 13: Structural Biology in Cells by In-cell NMRp343-378ByY. Fleischmann;Y. FleischmannLaboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, CH-8093 Zürich, SwitzerlandSearch for other works by this author on:J. Gerez;J. GerezLaboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, CH-8093 Zürich, SwitzerlandSearch for other works by this author on:R. RiekR. RiekLaboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, CH-8093 Zürich, SwitzerlandSearch for other works by this author on:
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