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This chapter introduces the topic of CD- (carbon dot) related imaging and therapy, beginning from a simple description of their preparation. CDs can be prepared with simple procedures from abundant precursors. These preparations produce composites, core-shell structures, and thus properties of CDs related to the bio-application. We further discuss the applications of CDs, such as cell imaging, including cytoplasm and organelle-targeted imaging, the multi-response from CDs and CD composites, imaging-guided photo-therapy, and the imaging-guided delivery system.

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