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The combination of Abacavir (ABA) and lamivudine (LAM) is being used in the treatment of HIV. Though, there was several spectroscopic methods have been reported, it needs a simple, sensitive, and rapid analytical method for precise analysis. The objective of the current research was to establish a UV-spectrophotometric method for simultaneous estimation of in their bulk and tablet dosage form. In the current study, both drugs were estimated by simultaneous equation method. The method is characterized by measurement of absorbances at two wavelengths 218 nm and 274 nm, λmax of ABA and LAM, respectively. The analysis was carried by Acetonitrile: water (50:50 %v/v) using UV-Visible Spectrophotometer (Eppendorf Biospectrometer Basic) with a fixed 2 nm spectral bandwidth. The Beer’s law was compiled in the concentrations ranging from 2.5-15 μm/mL and 2.5-12.5 μm/mL for ABA and LAM, respectively. The linear regression was found with correlation coefficient (r2) of 0.9935, 0.9825 for ABA and LAM, respectively. This study was performed as per ICH guidelines and various parameters were validated. The % RSD for accuracy and precision were lies within the acceptance criteria. The percentage (%) recovery was within the potency range (99.6-101.8%). This statistical data analysis proved that the method followed was accurate, rigorous, and reproducible for ABA and LAM analysis in their tablet dosage forms. The wide linearity range, sensitivity and simple procedure imply that the proposed technique demonstrated to more appropriate for the testing and analysis of tablet dosage forms.

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