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The development of reliable Fourier transform infrared (FTIR) instrumentation has made IR spectroscopy a more widely used technique for quantitative analysis. Qualitative organic compound identification has always been its strength. IRB and HTZ were estimated simultaneously in pharmaceutical dosage forms using a new FTIR spectroscopic approach. The approach involves KBr pelletization of IRB and HTZ and direct measurement with a reduced path length cell. In absorbance mode, the instrument gathered infrared spectra with 8cm-1 resolution. IR peaks changed after baseline adjustment. The strong, unambiguous, and proportionate carbonyl (C=O) and amine (N-H) functional groups of IRB and HTZ at 1733cm-1 and 3361cm-1 were chosen for quantitative measurement. IRB concentrations from 60-300μg/mg and HTZ concentrations from 5-25μg/mg followed Beer Lambert’s law. ICH guidelines validated the methodology. A statistical t-test was used to compare the results of the new FTIR method to the outcomes of the current UV method, and the results showed no statistically significant difference between the two ways at p=0.05. From the current study’s findings, it can be inferred that the simultaneous estimation method for IRB and HTZ is simple, accurate, and cost-effective. The proposed method can be successfully used to estimate IRB and HTZ simultaneously in pure and pharmaceutical dosage forms.

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