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Ancient times onwards mankind is dependent on the plants and other natural resources for the new chemical molecules in search of beneficial phytochemicals for therapy. Present study was intended to find out such phytochemicals in the ethanolic extract of Pseuderanthemum malabaricum which may become useful molecules in pharmacological perspective. Shade dried whole plant of Pseuderanthemum malabaricum was powdered and extracted with ethanol using Soxhlet extractor. Preliminary phytochemical analysis of ethanolic extract showed the presence of steroids, terpenoids, saponins, coumarins, flavonoids, phenols and chalcones. Gas chromatography-Mass spectroscopy/Mass spectroscopy (GC-MS/MS) analysis was carried out for identification and characterization of phytoconstituents by comparing with the database of National Institute Standard and Technology. Around 10 different types of chemical entities with varying quantities were identified. Among them, 4H-Pyran-4-one-2, 3-dihydro-3, 5-dihydroxy-6-methyl-(DDMP), 2-Furancarboxaldehyde-5 (hydroxymethyl) - (5HMF), 1-(+)-ascorbic acid-2-6- dihexadecanoate possess antioxidant, and anticancer properties. This stydy, identification of different biologically active compounds in the Pseuderanthemum malabaricum ethanolic extract forms the basis for further biological and pharmacological studies.

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