Chapter 3: Glycoprotein Characterization by Nuclear Magnetic Resonance Spectroscopy

Recent advances in glycoprotein expression systems and labelling strategies allow extending the application of nuclear magnetic resonance (NMR) to an increasing number of glycoproteins. In this context, NMR provides information about the exact stereochemistry of a glycan and the type of glycosidic linkages present in a sample and, therefore, is an excellent complement to mass spectrometry (MS). In this chapter, both the protein production methods for NMR studies and the different approaches to obtaining information about glycoproteins using NMR are described.