Chapter 6: AIE-based Fluorescence Imaging

The aggregation-induced emission (AIE) effect has emerged as a promising strategy to overcome the fluorescence quenching observed in traditional fluorescent probes in aggregate states (known as the ACQ effect). This breakthrough phenomenon holds great significance for enhancing fluorescence bioimaging techniques and advancing chemical biology. In this chapter, we present a concise overview of the working mechanism underlying the AIE effect. Furthermore, we highlight the applications of AIE-based fluorescence imaging in biomolecular sensing, as well as in cellular and subcellular bioimaging. The primary objective of this chapter is to provide the reader with a fundamental understanding of AIEgen construction and the selection of suitable AIE probes for specific bioimaging purposes. By arousing the reader’s interest, we aim to facilitate collaborative efforts in promoting the development of AIE techniques in chemical biology.