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Biomass-based carbon dots (BCDs) are carbon-based nanoparticles obtained by a series of syntheses using biomass as raw material, which have excellent chemical, physical and biological properties. The unique electronic structure of biomass carbon dots gives them excellent photoluminescence properties and they can be used for fluorescence bioimaging. This chapter introduces the latest progress in BCDs as a new multifunctional biomaterial, focusing on the source of raw materials and conversion mechanism of BCDs. The application progress of BCDs in bioimaging is reviewed, and the current limitations and future development directions of this exciting material are discussed.

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