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The detection and quantification of biomarkers using surface-enhanced Raman scattering (SERS)-based sensors have gained significant attention as a technology with enormous potential to transform diagnostics and personalised medicine. The concepts, design, and uses of SERS-based sensors in the context of biomarker analysis are thoroughly explored in this chapter. By concentrating on the plasmonic and chemical enhancement processes that underlie its exceptional sensitivity and specificity, we first clarify the essential concepts of SERS. The chapter further explores the complexities of the design of SERS sensors, highlighting the different metal nanostructures and the manufacturing methods used to enhance sensing performance. The benefits and distinctive qualities of SERS are highlighted through a thorough comparison with traditional detection techniques. Despite its immense promise, the assessment notes the difficulties with repeatability and practical applicability and provides suggestions on how to overcome these obstacles.

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