Peroxynitrite Detection in Biological Media: Challenges and Advances
CHAPTER 11: Reversible Near-Infrared Fluorescent Probes for Peroxynitrite Monitoring
Published:16 Oct 2015
Special Collection: 2015 ebook collection , 2011-2015 biosciences subject collectionSeries: Detection Science
Peng Li, Keli Han, 2015. "Reversible Near-Infrared Fluorescent Probes for Peroxynitrite Monitoring", Peroxynitrite Detection in Biological Media: Challenges and Advances, Serban Peteu, Sabine Szunerits, Mekki Bayachou
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Peroxynitrite (ONOO−, PN) is a biologically reactive species that is generated by the coupling reaction of nitric oxide (˙NO) and superoxide (O2˙−) radicals at diffusion-controlled rates of ∼10 M−1 s−1. The complex oxidation and nitration biology of PN and their broad implications in human disease and health provide motivation for developing new methods to detect PN in living systems. Several fluorescent probes have been synthesized and applied to detect PN in living cells. One disadvantage however is their relatively short excitation and emission wavelengths (∼550 nm), which render them difficult to employ for sensing and imaging PN in deep tissues and living animals. The other major drawback of these probes is their irreversibility in responding to PN. To solve these problems, near-infrared, reversible fluorescent probes have been developed that selectively respond to PN over other reactive oxygen species. This chapter reviews the design, synthesis, characterization and imaging applications of two cyanine-based, selenium/tellurium modulated fluorescent probes for PN.