Peroxynitrite Detection in Biological Media: Challenges and Advances
CHAPTER 6: Real Time Monitoring of Peroxynitrite by Stimulation of Macrophages with Ultramicroelectrodes
Published:16 Oct 2015
Special Collection: 2015 ebook collection , 2011-2015 biosciences subject collectionSeries: Detection Science
Christian Amatore, Manon Guille-Collignon, Frédéric Lemaître, 2015. "Real Time Monitoring of Peroxynitrite by Stimulation of Macrophages with Ultramicroelectrodes", Peroxynitrite Detection in Biological Media: Challenges and Advances, Serban Peteu, Sabine Szunerits, Mekki Bayachou
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In the two last decades, electrochemical techniques have been shown to be an efficient tool to investigate oxidative stress and the production of reactive oxygen and nitrogen species. Among them, the “artificial synapse” configuration involving platinized carbon fiber UMEs is of particular interest because it allows one to quantify in real time the very harmful and unstable peroxynitrite anion within the oxidative burst at the single cell level. In this chapter, the main studies dealing with this method of electrochemical detection of peroxynitrite will be summarized and commented upon. Additionally, because some drawbacks may remain, recent works devoted to the use of microsystems or nanotools will also be considered.