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Issues in Toxicology
The Comet Assay in Toxicology
Edited by
Alok Dhawan
Alok Dhawan
Centre of Biomedical Research, Lucknow, India
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Hardback ISBN:
978-1-78262-287-1
PDF ISBN:
978-1-78262-289-5
EPUB ISBN:
978-1-78262-915-3
Special Collection:
2016 ebook collection
Series:
Issues in Toxicology
Published online:
11 Oct 2016
Published in print:
07 Oct 2016
Book Chapter
Chapter 14: The Alkaline Comet Assay in Prognostic Tests for Male Infertility and Assisted Reproductive Technology Outcomes
By
Sheena E. M. Lewis
;
Sheena E. M. Lewis
Reproductive Medicine, Queen's University of Belfast, Institute of Clinical Science
Grosvenor Road
Belfast BT12 6BJ
Northern Ireland
UK
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Ishola M. Agbaje
Ishola M. Agbaje
Reproductive Medicine, Queen's University of Belfast, Institute of Clinical Science
Grosvenor Road
Belfast BT12 6BJ
Northern Ireland
UK
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Published:07 Oct 2016
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Special Collection: 2016 ebook collectionSeries: Issues in Toxicology
Page range:
369 - 389
Citation
S. E. M. Lewis and I. M. Agbaje, in The Comet Assay in Toxicology, ed. D. Anderson and A. Dhawan, The Royal Society of Chemistry, 2nd edn, 2016, ch. 14, pp. 369-389.
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In this review the relationship between actual sperm DNA damage as determined by the alkaline Comet assay and potential DNA damage as measured by DNA adduct testing will also be examined and the potential of routine clinical practices such as cryopreservation and prolonged incubation to induce further DNA damage investigated. The usefulness of sperm DNA tests as prognostic markers and in particular, the opportunities and challenges provided by DNA testing in male-fertility determination will be discussed.
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