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The in vivo mammalian alkaline Comet assay is used for assessing the DNA-damaging potential of chemicals in cells and tissues of rats and mice. The assay has been adopted by the OECD, as a second-tier test, in the standard battery of genotoxicity tests. This chapter outlines the protocol followed for performing the in vivo Comet assay in cells of blood and different organs e.g. liver, kidney, brain, spleen and bone marrow of mouse.

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