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Among the manifold advantages of the Comet assay is the ability to evaluate very limited amounts of test agents. In many cases compounds for a research study are not commercially available and the chemicals must be individually synthesised at great expense and with large amounts of time involved. In this chapter we shall describe some of our work with a series of high-priority nitrogen-containing drinking-water disinfection byproducts (DBPs) that were synthesised as high-purity chemical analytical standards. The use of 96-well microplates allowed us to analyse the genotoxicity of these DBPs in mammalian cells.

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