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Issues in Toxicology
The Comet Assay in Toxicology
Edited by
Alok Dhawan
Alok Dhawan
Centre of Biomedical Research, Lucknow, India
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Hardback ISBN:
978-1-78262-287-1
PDF ISBN:
978-1-78262-289-5
EPUB ISBN:
978-1-78262-915-3
Special Collection:
2016 ebook collection
Series:
Issues in Toxicology
Publication date:
07 Oct 2016
Book Chapter
Chapter 5: Methods for Freezing Blood Samples at −80 °C for DNA Damage Analysis in Human Leukocytes
By
Narendra P. Singh
;
Narendra P. Singh
Department of Bioengineering
Box 355061, University of Washington
Seattle, WA 98195-5061
USA
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Henry C. Lai
Henry C. Lai
Department of Bioengineering
Box 355061, University of Washington
Seattle, WA 98195-5061
USA
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Published:07 Oct 2016
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Special Collection: 2016 ebook collectionSeries: Issues in Toxicology
Page range:
134 - 142
Citation
N. P. Singh and H. C. Lai, in The Comet Assay in Toxicology, ed. D. Anderson and A. Dhawan, The Royal Society of Chemistry, 2nd edn, 2016, ch. 5, pp. 134-142.
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It was hypothesised that certain freezing protocols would more fully reveal DNA damage induced by chemical or physical agents. X-Ray induced DNA damage was used as a model for DNA damage induced by exposure to genotoxic agents. Here, we report two new protocols for the assessment of DNA-damage levels in human whole blood nucleated cells.
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