The Comet Assay in Toxicology
Chapter 5: Methods for Freezing Blood Samples at −80 °C for DNA Damage Analysis in Human Leukocytes
Published:07 Oct 2016
It was hypothesised that certain freezing protocols would more fully reveal DNA damage induced by chemical or physical agents. X-Ray induced DNA damage was used as a model for DNA damage induced by exposure to genotoxic agents. Here, we report two new protocols for the assessment of DNA-damage levels in human whole blood nucleated cells.