CHAPTER 2: Introduction to Structural Studies on 2-Oxoglutarate-Dependent Oxygenases and Related Enzymes
Published:23 Apr 2015
Wei Shen Aik, Rasheduzzaman Chowdhury, Ian J. Clifton, Richard J. Hopkinson, Thomas Leissing, Michael A. McDonough, Radosław Nowak, Christopher J. Schofield, Louise J. Walport, 2015. "Introduction to Structural Studies on 2-Oxoglutarate-Dependent Oxygenases and Related Enzymes", 2-Oxoglutarate-Dependent Oxygenases, Christopher Schofield, Robert Hausinger
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The first Fe(ii)- and 2-oxoglutarate (2OG)-dependent oxygenases to be identified were the animal pro-collagen hydroxylases (see Chapter 5).1–3 However, to date the oxygenase domains of these enzymes have proven to be rather recalcitrant with respect to high-resolution structural studies. Instead, the first crystal structure of a member of this superfamily (henceforth simplified to the 2OG oxygenases) came from work on enzymes involved in the biosynthesis of the β-lactam antibiotics.4–6 This work was enabled by contemporary advances in molecular biology, which allowed the production of unnaturally large amounts of protein in recombinant hosts. The first structure of...