2-Oxoglutarate-Dependent Oxygenases
CHAPTER 2: Introduction to Structural Studies on 2-Oxoglutarate-Dependent Oxygenases and Related Enzymes
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Published:23 Apr 2015
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Wei Shen Aik, Rasheduzzaman Chowdhury, Ian J. Clifton, Richard J. Hopkinson, Thomas Leissing, Michael A. McDonough, Radosław Nowak, Christopher J. Schofield, Louise J. Walport, 2015. "Introduction to Structural Studies on 2-Oxoglutarate-Dependent Oxygenases and Related Enzymes", 2-Oxoglutarate-Dependent Oxygenases, Christopher Schofield, Robert Hausinger
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The first Fe(ii)- and 2-oxoglutarate (2OG)-dependent oxygenases to be identified were the animal pro-collagen hydroxylases (see Chapter 5).1–3 However, to date the oxygenase domains of these enzymes have proven to be rather recalcitrant with respect to high-resolution structural studies. Instead, the first crystal structure of a member of this superfamily (henceforth simplified to the 2OG oxygenases) came from work on enzymes involved in the biosynthesis of the β-lactam antibiotics.4–6 This work was enabled by contemporary advances in molecular biology, which allowed the production of unnaturally large amounts of protein in recombinant hosts. The first structure of...