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This chapter focuses on the problem of detecting, characterizing, and determining the concentration of nanomaterials in foods and other biological matrices. After providing an overview of the unique challenges associated with nanoparticle metrology in complex media, sample pretreatment methods (including extraction, digestion, and inline chromatographic separation), imaging analysis, and nanomaterial quantification methods are presented in detail. The chapter also addresses numerous methods under development, including atmospheric scanning electron microscopy, single-particle inductively coupled plasma mass spectrometry, immunological detection methods, and optical techniques such surface plasmon resonance. The chapter concludes with an overview of the research needs in this area.

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