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The high sensitivity and spatio-temporal resolution of positron emission tomography (PET) makes this non-invasive imaging technique ideal for the in vivo tracking of liposomal nanomedicines in the clinical setting. This image-guided therapeutic approach may eventually allow selection of patients that would benefit from the nanomedicinal treatment and, by doing so, enhance the clinical value/efficacy of this promising treatment. In this chapter, we review the different methods reported to date of radiolabel liposomal nanomedicines with PET isotopes such as 89Zr, 64Cu and 18F and discuss their advantages and limitations for clinical translation.

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