High Throughput Screening Methods: Evolution and Refinement
High throughput screening remains a key part of early stage drug and tool compound discovery, and methods and technologies have seen many fundamental improvements and innovations over the past 20 years. This comprehensive book provides a historical survey of the field up to the current state-of-the-art. In addition to the specific methods, this book also considers cultural and organizational questions that represent opportunities for future success.
Following thought-provoking foreword and introduction from Professor Stuart Schreiber and the editors, chapters from leading experts across academia and industry cover initial considerations for screening, methods appropriate for different goals in small molecule discovery, newer technologies that provide alternative approaches to traditional miniaturization procedures, and practical aspects such as cost and resourcing. Within the context of their historical development, authors explain common pitfalls and their solutions.
This book will serve as both a practical reference and a thoughtful guide to the philosophy underlying technological change in such a fast-moving area for postgraduates and researchers in academia and industry, particularly in the areas of chemical biology, pharmacology, structural biology and assay development.
High Throughput Screening Methods: Evolution and Refinement, The Royal Society of Chemistry, 2016.
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Table of contents
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Chapter 1: HTS Methods: Assay Design and Optimisationp1-15ByMark WigglesworthMark WigglesworthSearch for other works by this author on:
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Chapter 2: Considerations Related to Small-molecule Screening Collectionsp16-36ByDamian W. YoungDamian W. YoungDepartment of Pharmacology and Pathology and Immunology, Center for Drug Discovery, Baylor College of MedicineOne Baylor PlazaHoustonTexas77030USA[email protected]Search for other works by this author on:
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Chapter 3: Combination Screeningp37-57ByJohn Moffat;John MoffatDepartment of Biochemical and Cellular Pharmacology, Genentech Research & Early DevelopmentSouth San FranciscoUSASearch for other works by this author on:Mats GustafssonMats GustafssonSearch for other works by this author on:
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Chapter 4: Modern Biophysical Methods for Screening and Drug Discoveryp58-86ByB. Fulroth;B. FulrothCenter for the Development of TherapeuticsThe Broad Institute of MIT and Harvard 415 Main St.CambridgeMA 02421USA[email protected]Search for other works by this author on:V. K. Kaushik;V. K. KaushikCenter for the Development of TherapeuticsThe Broad Institute of MIT and Harvard 415 Main St.CambridgeMA 02421USA[email protected]Search for other works by this author on:M. F. MeslehM. F. MeslehCenter for the Development of TherapeuticsThe Broad Institute of MIT and Harvard 415 Main St.CambridgeMA 02421USA[email protected]Search for other works by this author on:
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Chapter 5: Genetic Perturbation Methods, from the ‘Awesome Power’ of Yeast Genetics to the CRISPR Revolutionp87-116ByGregory R. Hoffman;Gregory R. HoffmanNovartis Institutes for BioMedical Research250 Massachusetts AvenueCambridge, MA 02139USA[email protected]Search for other works by this author on:Dominic HoepfnerDominic HoepfnerSearch for other works by this author on:
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Chapter 6: Understanding Luminescence Based Screensp117-142BySimona Gokhin;Simona GokhinCenter for Proteomic Chemistry Novartis Institutes for Biomedical Research250 Massachusetts AvenueCambridge, MA 02139USA[email protected]Search for other works by this author on:Douglas S. AuldDouglas S. AuldCenter for Proteomic Chemistry Novartis Institutes for Biomedical Research250 Massachusetts AvenueCambridge, MA 02139USA[email protected]Search for other works by this author on:
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Chapter 7: High Throughput Screening Compatible Methods for Quantifying Protein Interactions in Living Cellsp143-161ByK. V. WoodK. V. WoodSearch for other works by this author on:
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Chapter 8: Approaches to High Content Imaging and Multi-feature Analysisp162-180ByD. NojimaD. NojimaSearch for other works by this author on:
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Chapter 9: Pharmacological and Genetic Screening of Molecularly Characterized Cell Linesp181-213ByZhaleh Safikhani;Zhaleh SafikhaniDepartment of Medical Biophysics, University of TorontoTorontoONCanadaSearch for other works by this author on:Heather Selby;Heather SelbyDana-Farber Cancer Institute, Harvard Medical SchoolBostonMAUSADepartment of Bioinformatics, Boston UniversityBostonMAUSASearch for other works by this author on:Christos Hatzis;Christos HatzisYale School of Medicine, Yale UniversityNew HavenConnecticutUSAYale Cancer CenterNew HavenConnecticutUSASearch for other works by this author on:Benjamin Haibe-KainsBenjamin Haibe-KainsDepartment of Medical Biophysics, University of TorontoTorontoONCanadaDepartment of Computer Science, University of TorontoTorontoONCanadaOntario Institute of Cancer ResearchTorontoONCanadaSearch for other works by this author on:
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Chapter 10: Multidimensional Profile Based Screening: Understanding Biology through Cellular Response Signaturesp214-238ByChristopher C. Mader;Christopher C. MaderThe Broad Institute of MIT and Harvard, Cancer Program415 Main Street, Cambridge, MA 02142USA[email protected][email protected]Search for other works by this author on:Aravind Subramanian;Aravind SubramanianThe Broad Institute of MIT and Harvard, Cancer Program415 Main Street, Cambridge, MA 02142USA[email protected][email protected]Search for other works by this author on:Joshua BittkerJoshua BittkerThe Broad Institute of MIT and Harvard, Center for Development of Therapeutics415 Main StreetCambridgeMA 02142USASearch for other works by this author on:
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Chapter 11: 3D Cell Culture and Dish Based Organogenesis: Optimizing In vitro Cellular Physiologyp239-251ByYe Fang;Ye FangBiochemical Technologies, Science and Technology Division, Corning IncorporatedCorningNY 14831USASearch for other works by this author on:Richard M. EglenRichard M. EglenSearch for other works by this author on:
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Chapter 12: Small-molecule-mediated Targeted Protein Degradation for Drug Discoveryp252-274ByRohan E. J. BeckwithRohan E. J. BeckwithNovartis Institutes for BioMedical Research, Global Discovery Chemistry181 Massachusetts AvenueCambridge02139USA[email protected]Search for other works by this author on:
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Chapter 13: Phenotypic Screens with Model Organismsp275-302ByPeixin Zhu;Peixin ZhuNovartis Institutes for Biomedical Research, Dept. of Developmental & Molecular Pathways181 Massachusetts AvenueCambridge02139USA[email protected][email protected]Harvard University, Stem Cell & Regenerative Biology7 Divinity AvenueCambridgeMA 02138USASearch for other works by this author on:Gerald J. Sun;Gerald J. SunNovartis Institutes for Biomedical Research, Dept. of Developmental & Molecular Pathways181 Massachusetts AvenueCambridge02139USA[email protected][email protected]Search for other works by this author on:Brant K. PetersonBrant K. PetersonNovartis Institutes for Biomedical Research, Dept. of Informatics181 Massachusetts AvenueCambridge02139USASearch for other works by this author on:
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Chapter 14: Encoded Compound Libraries to Accelerate Small-molecule Therapeutic Discoveryp303-323ByStephen P. HaleStephen P. HaleSearch for other works by this author on:
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Chapter 15: Research Data Managementp324-343ByShuba Gopal;Shuba GopalThe Broad Institute of MIT and HarvardCambridge, MAUSASearch for other works by this author on:Andrea de SouzaAndrea de SouzaSearch for other works by this author on:
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Chapter 16: Small-molecule Bioactivity Databasesp344-371BySean Ekins;Sean EkinsCollaborative Drug Discovery, Inc.1633 Bayshore HighwaySuite 342Burlingame, CA 94010USA[email protected]Collaborations Pharmaceuticals, Inc.5616 Hilltop Needmore RoadFuquay-Varina, NC 27526USASearch for other works by this author on:Alex M. Clark;Alex M. ClarkCollaborative Drug Discovery, Inc.1633 Bayshore HighwaySuite 342Burlingame, CA 94010USA[email protected]Molecular Materials Informatics, Inc.1900 St. Jacques #302, Montreal H3J 2S1QuebecCanadaSearch for other works by this author on:Christopher Southan;Christopher SouthanIUPHAR/BPS Guide to PHARMACOLOGY, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson BuildingEdinburgh, EH8 9XDUKSearch for other works by this author on:Barry A. Bunin;Barry A. BuninCollaborative Drug Discovery, Inc.1633 Bayshore HighwaySuite 342Burlingame, CA 94010USA[email protected]Search for other works by this author on:Antony J. WilliamsAntony J. WilliamsChemConnector513 Chestnut Grove CourtWake Forest, NC 27587USASearch for other works by this author on:
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Chapter 17: “So You Want to Run a High-throughput Screen: Do You Know How Much That Costs?”; Costs of High Throughput Screens and How to Fund Themp372-389ByJoshua A. BittkerJoshua A. BittkerCenter for the Development of Therapeutics, The Broad Institute of MIT and HarvardCambridge, MAUSASearch for other works by this author on:
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