Protein glycosylation plays essential roles in regulating physiological activities of proteins. Visualization of protein glycans in living system is invaluable for elucidating the biological functions of protein glycosylation. However, it has proven challenging to develop tools for molecular imaging of glycans. This chapter highlights recently developed chemical tools that enable visualization of protein glycosylation. The development of these chemical methods is introduced in the contexts of important protein glycosylation and imaging tools for visualizing glycans in general.