The Biological Chemistry of Nickel
CHAPTER 17: Application of Ni2+-Binding Proteins
Published:24 Mar 2017
J. Zakrzewska-Czerwińska, A. M. Zawilak-Pawlik, 2017. "Application of Ni2+-Binding Proteins", The Biological Chemistry of Nickel, Deborah Zamble, Magdalena Rowińska-Żyrek, Henryk Kozlowski
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Proteins that bind nickel play important roles in various biological processes. They are either metalloenzymes, whose activity depends on Ni2+, or processing proteins, responsible for Ni2+ sensing, uptake, and storage, because free transition metals are harmful to the cell. However, nickel-binding proteins, or their fragments, are also considered biomolecules, which may be applied in science, medicine, or biotechnology. In fact, the histidine polypeptide (His-tag) is an example of one of the most successful applications of the nickel-binding motif in molecular biology. There are many other ways to exploit nickel-binding proteins as biosensors, drug targets, or in remediation technology.