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One of the most daunting challenges when studying lignin relates to analytical chemistry approaches applied to the heterogeneous polymer or depolymerization products. Understanding the structure of lignin in the cell wall alone is a target that remains incredibly challenging. This chapter will cover advances in understanding lignin in planta as well as characterizing isolated lignin or lignin depolymerization products, highlighting the methods and their respective advantages and disadvantages in terms of reliable data acquisition.

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