Chapter 31: EPR Detection (Spin Probes)
Published:27 Jan 2016
Special Collection: 2016 ebook collection
É. Hideg, A. Mátai, B. Bognár, and T. Kálai, in Singlet Oxygen: Applications in Biosciences and Nanosciences, ed. S. Nonell, C. Flors, S. Nonell, and C. Flors, The Royal Society of Chemistry, 2016, ch. 31, pp. 121-134.
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Singlet oxygen is a unique molecule among reactive oxygen species, with equally fascinating physicochemical and photobiological features. In this chapter we review the principles and biological applications of its detection using spin trapping electron paramagnetic resonance (EPR) spectroscopy. In addition to introducing the chemistry behind the technique itself, possible applications are illustrated using examples from both medicinal and plant biochemistry.