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New Developments in NMR
NMR in Glycoscience and Glycotechnology
Edited by
Koichi Kato;
Koichi Kato
National Institutes of Natural Sciences, Japan
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Thomas Peters
Thomas Peters
University of Luebeck, Germany
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Hardback ISBN:
978-1-78262-310-6
PDF ISBN:
978-1-78262-394-6
EPUB ISBN:
978-1-78801-128-0
Special Collection:
2017 ebook collection
Series:
New Developments in NMR
No. of Pages:
402
Published online:
15 May 2017
Published in print:
17 May 2017
Book Chapter
CHAPTER 9: Quantifying Carbohydrate Motions Through Solution Measurements: Applications to Immunoglobulin G Fc
By
Adam W. Barb
Adam W. Barb
Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University
Ames
IA 50011
USA
[email protected]
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Published:15 May 2017
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Special Collection: 2017 ebook collectionSeries: New Developments in NMR
Page range:
208 - 227
Citation
A. W. Barb, in NMR in Glycoscience and Glycotechnology, ed. K. Kato and T. Peters, The Royal Society of Chemistry, 2017, pp. 208-227.
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This chapter describes the preparation of homogenous N-glycoproteins using in vivo or in vitro manipulation with a special emphasis on incorporating stable isotope labels for inspection by solution NMR spectroscopy. This also includes an introduction to basic solution NMR techniques for characterizing glycoprotein motion. The power of these techniques is highlighted by an analysis of recent breakthroughs in the characterization of the structure/activity relationship of antibody N-glycosylation with a discussion of future opportunities in this area of structural biology.
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