NMR in Glycoscience and Glycotechnology
CHAPTER 8: Stable Isotope Labeling of Glycoproteins for NMR Study
Published:15 May 2017
Special Collection: 2017 ebook collectionSeries: New Developments in NMR
Y. Yamaguchi, H. Yagi, and K. Kato, in NMR in Glycoscience and Glycotechnology, ed. K. Kato and T. Peters, The Royal Society of Chemistry, 2017, pp. 194-207.
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In the study of glycoproteins by NMR spectroscopy, stable isotope labeling is a challenging but important step. Labeling by metabolic means is useful, but choice of production vehicles (mammalian or non-mammalian expression systems) and of medium (or diet) are critical for obtaining functional glycoproteins with high isotope enrichment and with the appropriate glycoform framework. Metabolic labeling coupled with in vitro enzymatic reactions enables remodeling or modification of the NMR target glycoform. This chapter outlines recent progress in isotope labeling techniques of glycoproteins using mammalian and non-mammalian expression systems.