CHAPTER 15: SynBiocat: Protein Purification, Immobilization and Continuous-flow Processes

The activity of SynBiocat Ltd, a small spin-off company, is related to various areas of synthetic chemistry, biotechnology and enzymology. This chapter gives an overview of the knowledge and technologies of the company covering the development of suitable biocatalysts and bioreactor solutions for synthetic biotransformations. In co-operation with Bioorganic Chemistry Group (BCG) at Budapest University of Technology and Economics and Fermentia Ltd, SynBiocat can offer a wide selection of enzyme immobilization methods for known or novel enzymes to select the most suitable variant for a desired technology. Our company also offers custom immobilization development as a service to develop the optimal immobilized form of the biocatalyst of customers. For immediate screening, kits of immobilization supports or immobilized enzymes such as lipases, proteases, ketoreductases (as whole-cell biocatalysts), phenylalanine ammonia-lyases, phenylalanine 2,3-aminomutases are accessible. To enable screening of continuous-flow biotransformations, a selection of immobilized biocatalyst-filled minireactors are available off-the-shelf.