The biocatalyst market is anticipated to see high growth rates in many different areas and applications for the foreseeable future. This chapter provides insight into biocatalysis applications in the food & beverage industry, pharmaceutical industry and antibiotic industry. Recent advances, challenges, future perspectives and applications of UDP-glycosyltransferase, ketoreductase, cephalosporin C acylase and deacetoxycephalosporin C synthase are discussed in detail in order to give the reader insight into screening, enzyme engineering, biotransformation and scaling up processes for manufacturing.