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The ability of enzymes to catalyze chemical reactions with high rate acceleration (kcat/kuncat=107–1026)1,2  and unmatched selectivity makes them highly attractive for use as biocatalysts in a large number of industrial syntheses. However, the finite repertoire of naturally-occurring enzyme activities and the fact that few enzymes inherently catalyze desired reactions under industrially-relevant conditions restrict their applicability in many industrial processes. To make enzymes practical for industrial syntheses, it is often necessary to tailor their catalytic activity to the intended application using protein engineering. Protein engineering techniques such as rational design...

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