Stevia rebaudiana belongs to the Asteraceae family of plants and it is native to Paraguay. Stevia especially is known with its high sweetening property due to ent-kaurene glycosides present in the leaves.¹  While there are several studies with steviol glycosides²  and poylphenolic secondary metabolites^3,4  of stevia in the literature, there is a single study reporting its lipids content⁵  and one contribution describing its volatile terpenes.⁶  To allow a full chemical characterisation of stevia leaves a thorough profiling of its lipids was carried out. This chapter represents a summary of original research that has not been previously published in peer-reviewed journals.