Overuse of agrochemicals has become of increasing concern because they negatively affect many non-target organisms, particularly amphibians. In this chapter, we support the use of the direct-developing frog Eleutherodactylus johnstonei as an innovative and promising model organism in terrestrial ecotoxicology. Standard guidelines for collecting, housing, and rearing this frog under laboratory conditions are given. We also provide an overview of candidate end-points and ecotoxicological tools for the future use of this species in ecotoxicological studies. Further ecotoxicological research focused on E. johnstonei as a model organism would be a welcome addition to our understanding of agrochemical exposure in terrestrial amphibians.