CHAPTER 15: Intensified Production of Recombinant Proteins

Recent advances in the production of recombinant proteins have led to significant process improvements. In this chapter, novel techniques involving continuous or semi-continuous steady state upstream and downstream processing are described. In the upstream part, continuous perfusion bioreactors enable large reductions in reactor volumes and increases in product quality by assuring steady state operations. In the downstream part, countercurrent chromatographic processes for both capture and polishing increase separation yield and product, and allow for faster and more efficient processing of the proteins. Together, these developments significantly increase the yield of protein production, as well as reduce the footprint of the entire process.