CHAPTER 17: Micronucleus Experiments with Bivalve Molluscs

The micronucleus (MN) test, as an index of accumulated DNA damage during the lifespan of cells, is the most applied assay in aquatic animals to assess the exposure to a complex mixture of genotoxic pollutants. An increase in MN frequency was reported on mussels exposed to the most common environmental pollutants under laboratory conditions, such as heavy metals, polycyclic aromatic hydrocarbons, and ionizing radiation. The test was applied in a large number of biomonitoring studies in different geographic areas to identify the exposure to different classes of pollutants with good discrimination power and to evaluate the recovery effects after accidental pollution events. A standardized MN assay protocol in hemocytes and gill cells for use in bivalve species, including scoring of different cell types, necrotic and apoptotic cells and nuclear anomalies, was established following the “cytome approach”. The mussel MNcytome (MUMNcyt) assay, using the proposed detailed criteria for the identification of cell types, is suitable for application in experimental studies under controlled conditions and in biomonitoring programs in aquatic environments.