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As optoelectronics and microelectronics have evolved, new alternatives in phosphorescence measurement instrumentation have been recently proposed. In this chapter we present the phosphorescent system as a linear system (described by a linear differential equation), whose parameters depend on the oxygen concentration to be determined. Under this perspective, the flexibility for exciting the sensing phase with a modulated light source and the signal processing applied to the registered luminescent signals can be exploited in order to optimize the information provided by the phosphorescence instrument. These concepts allow the design of new low-cost and flexible instruments specifically oriented to different purposes, such as: (a) optimal characterization of the sensing phase; (b) improved accuracy of the oxygen determination; (c) characterization of the background noise in order to provide a confidence interval together with the oxygen determination; or (d) integrating information from several excitation frequencies for a single accurate oxygen determination. In this chapter we describe the principles of this new perspective and how these concepts can be integrated in a phosphorescence instrument.

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