Field-cycling NMR Relaxometry: Instrumentation, Model Theories and Applications
Chapter 15: High-resolution Applications of Shuttle Field-cycling NMR
Published:11 Oct 2018
High-resolution shuttle field-cycling relaxometry has proven to be a robust technique for characterizing 31P in biological molecules, particularly phospholipids. Instrument and sample requirements and constraints for the Redfield “spin spa”, typical R1 profiles covering 0.002–11.74 T and the relaxation mechanisms responsible for different features are reviewed. With this as background, results analyzing several biological systems are examined to highlight how different parameters extracted from the data can be used to measure the dynamics of ligands bound to proteins, emphasizing the use of paramagnetic relaxation enhancement (PR1E) at low fields to identify novel moderate-affinity sites for phospholipids on amphitropic proteins.