In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology
In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.
Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.
In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology, The Royal Society of Chemistry, 2019.
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Table of contents
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Chapter 1: In-cell NMR Spectroscopy: From 2001 to Nowp1-9ByVolker DötschVolker DötschSearch for other works by this author on:
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Chapter 2: Protein NMR in Escherichia colip11-26ByYansheng Ye;Yansheng YeKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of SciencesWuhanHubei 430071Chinaconggangli@wipm.ac.cnSearch for other works by this author on:Conggang LiConggang LiKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of SciencesWuhanHubei 430071Chinaconggangli@wipm.ac.cnSearch for other works by this author on:
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Chapter 3: In-cell NMR in Eukaryotic Cells – I (Introduction from Outside of Cells)p27-44ByKohsuke Inomata;Kohsuke InomataRIKEN Center for Biosystems Dynamics Research1-7-22 Suehiro-choTsurumi-kuYokohama 230-0045Japankinomata@riken.jpSearch for other works by this author on:Takanori Kigawa;Takanori KigawaRIKEN Center for Biosystems Dynamics Research1-7-22 Suehiro-choTsurumi-kuYokohama 230-0045Japankinomata@riken.jpDepartment of Computer Science, School of Computing, Tokyo Institute of Technology4259 Nagatsuta-choMidori-ku, YokohamaKanagawa 226-8503JapanSearch for other works by this author on:Masahiro ShirakawaMasahiro ShirakawaDepartment of Molecular Engineering, Graduate School of Engineering, Kyoto UniversityKyoto-Daigaku KatsuraNishikyo-KuKyoto 615-8510Japanshirakawa@moleng.kyoto-u.ac.jpSearch for other works by this author on:
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Chapter 4: In-cell NMR in Eukaryotic Cells – II (Direct Protein Expression)p45-61ByEnrico Luchinat;Enrico LuchinatMagnetic Resonance Center – CERM, University of Florence50019 SestoFiorentinoItalybanci@cerm.unifi.itDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence50134FlorenceItalySearch for other works by this author on:Lucia BanciLucia BanciMagnetic Resonance Center – CERM, University of Florence50019 SestoFiorentinoItalybanci@cerm.unifi.itDepartment of Chemistry, University of Florence50019 SestoFiorentinoItalySearch for other works by this author on:
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Chapter 5: Protein Structure Determination in Living Cells from NOE-derived Distance Restraintsp63-89ByTeppei Ikeya;Teppei IkeyaDepartment of Chemistry, Tokyo Metropolitan University1-1 Minami OsawaHachiojiTokyo 192-0397Japantikeya@tmu.ac.jpito-yutaka@tmu.ac.jpSearch for other works by this author on:Peter Güntert;Peter GüntertDepartment of Chemistry, Tokyo Metropolitan University1-1 Minami OsawaHachiojiTokyo 192-0397Japantikeya@tmu.ac.jpito-yutaka@tmu.ac.jpInstitute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt60438 Frankfurt am MainGermanyLaboratory of Physical Chemistry, ETH Zürich8093 ZürichSwitzerlandSearch for other works by this author on:Yutaka ItoYutaka ItoDepartment of Chemistry, Tokyo Metropolitan University1-1 Minami OsawaHachiojiTokyo 192-0397Japantikeya@tmu.ac.jpito-yutaka@tmu.ac.jpSearch for other works by this author on:
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Chapter 6: In-cell Structure Determination Using Paramagnetic NMR Spectroscopyp90-102ByXun-Cheng Su;Xun-Cheng SuState Key Laboratory of Elemento-organic Chemistry, Department of Chemical Biology, College of Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai UniversityTianjin 300071Chinaxunchengsu@nankai.edu.cnSearch for other works by this author on:Thomas HuberThomas HuberResearch School of Chemistry, Australian National UniversityCanberraACT 2601Australiat.huber@anu.edu.auSearch for other works by this author on:
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Chapter 7: Bioreactor System for In-cell NMR Observationp103-116ByNoritaka Nishida;Noritaka NishidaGraduate School of Pharmaceutical Sciences, The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japanshimada@iw-nmr.f.u-tokyo.ac.jpSearch for other works by this author on:Ichio ShimadaIchio ShimadaGraduate School of Pharmaceutical Sciences, The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japanshimada@iw-nmr.f.u-tokyo.ac.jpSearch for other works by this author on:
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Chapter 8: 19F In-cell NMRp117-130ByYansheng Ye;Yansheng YeKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of SciencesWuhanHubei 430071Chinaconggangli@wipm.ac.cnSearch for other works by this author on:Conggang LiConggang LiKey Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of SciencesWuhanHubei 430071Chinaconggangli@wipm.ac.cnSearch for other works by this author on:
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Chapter 9: Cellular Solid-state NMR Spectroscopyp131-151ByReinier Damman;Reinier DammanNMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University3584 CH UtrechtThe Netherlandsm.baldus@uu.nlSearch for other works by this author on:Siddarth Narasimhan;Siddarth NarasimhanNMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University3584 CH UtrechtThe Netherlandsm.baldus@uu.nlSearch for other works by this author on:Markus Weingarth;Markus WeingarthNMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University3584 CH UtrechtThe Netherlandsm.baldus@uu.nlSearch for other works by this author on:Marc BaldusMarc BaldusNMR Spectroscopy, Bijvoet Center for Biomolecular Research, Utrecht University3584 CH UtrechtThe Netherlandsm.baldus@uu.nlSearch for other works by this author on:
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Chapter 10: In-cell EPRp152-169ByAnna Bieber;Anna BieberDepartment of Chemistry and Research School Chemical Biology, University of Konstanz78457 KonstanzGermanymalte.drescher@uni–konstanz.deSearch for other works by this author on:Malte DrescherMalte DrescherDepartment of Chemistry and Research School Chemical Biology, University of Konstanz78457 KonstanzGermanymalte.drescher@uni–konstanz.deSearch for other works by this author on:
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Chapter 11: In-cell NMR: Spectral Optimization and Population Adjustment by Rational Protein Engineeringp171-187ByJens Danielsson;Jens DanielssonDepartment of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm UniversityS-106 91 StockholmSwedenjens.danielsson@dbb.su.semikael.oliveberg@dbb.su.seSearch for other works by this author on:Fan Yang;Fan YangDepartment of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm UniversityS-106 91 StockholmSwedenjens.danielsson@dbb.su.semikael.oliveberg@dbb.su.seSearch for other works by this author on:Mikael OlivebergMikael OlivebergDepartment of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm UniversityS-106 91 StockholmSwedenjens.danielsson@dbb.su.semikael.oliveberg@dbb.su.seSearch for other works by this author on:
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Chapter 12: Protein Stability and Weak Intracellular Interactionsp188-206ByAlex J. Guseman;Alex J. GusemanDepartment of Chemistry, University of North Carolina at Chapel HillChapel HillNorth Carolina 27599USAgary_pielak@unc.eduSearch for other works by this author on:Gary J. PielakGary J. PielakDepartment of Chemistry, University of North Carolina at Chapel HillChapel HillNorth Carolina 27599USAgary_pielak@unc.eduDepartment of Biochemistry & Biophysics, University of North Carolina at Chapel HillChapel HillNorth Carolina 27599USALineberger Comprehensive Cancer Center, University of North Carolina at Chapel HillChapel HillNorth Carolina 27599USAIntegrative Program for Biological and Genome Sciences, University of North Carolina at Chapel HillChapel HillNorth Carolina 27599USASearch for other works by this author on:
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Chapter 13: Protein Folding, Maturation and Redox Statep207-227ByEnrico Luchinat;Enrico LuchinatMagnetic Resonance Center – CERM, University of Florence50019 Sesto FiorentinoItalyeluchinat@cerm.unifi.itDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence50134 FlorenceItalySearch for other works by this author on:Lucia BanciLucia BanciMagnetic Resonance Center – CERM, University of Florence50019 Sesto FiorentinoItalyeluchinat@cerm.unifi.itDepartment of Chemistry, University of Florence50019 Sesto FiorentinoItalySearch for other works by this author on:
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Chapter 14: All-atom Molecular Dynamics Simulation of Proteins in Crowded Environmentsp228-248ByYuji Sugita;Yuji SugitaRIKEN Center for Computational Science6-7-1 Minatojima-minamimachiChuo-ku, KobeHyogo 640-0047JapanRIKEN Center for Biosystems Dynamics Research6-7-1 Minatojima-minamimachiChuo-ku, KobeHyogo 640-0047JapanSearch for other works by this author on:Michael FeigMichael FeigRIKEN Center for Biosystems Dynamics Research6-7-1 Minatojima-minamimachiChuo-ku, KobeHyogo 640-0047JapanDepartment of Biochemistry and Molecular Biology, Michigan State University603 Wilson Rd. Room 218 BCHEast LansingMichigan 48824USASearch for other works by this author on:
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Chapter 15: Primary Drug Screening by In-cell NMR Spectroscopyp249-271ByStephen Dansereau;Stephen DansereauDepartment of Chemistry, University at Albany, State University of New York1400 Washington Ave.AlbanyNY 12222USAashekhtman@albany.eduSearch for other works by this author on:David S. Burz;David S. BurzDepartment of Chemistry, University at Albany, State University of New York1400 Washington Ave.AlbanyNY 12222USAashekhtman@albany.eduSearch for other works by this author on:Alexander ShekhtmanAlexander ShekhtmanDepartment of Chemistry, University at Albany, State University of New York1400 Washington Ave.AlbanyNY 12222USAashekhtman@albany.eduSearch for other works by this author on:
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Chapter 16: In-cell NMR Spectroscopy of Nucleic Acidsp272-297BySimon Dzatko;Simon DzatkoCentral European Institute of Technology, Masaryk UniversityBrnoCzech Republiclukas.trantirek@ceitec.muni.czSearch for other works by this author on:Radovan Fiala;Radovan FialaCentral European Institute of Technology, Masaryk UniversityBrnoCzech Republiclukas.trantirek@ceitec.muni.czSearch for other works by this author on:Robert Hänsel-Hertsch;Robert Hänsel-HertschCenter for Molecular Medicine Cologne (CMMC), University of Cologne50931 CologneGermanyCologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne50931 CologneGermanySearch for other works by this author on:Silvie Foldynova-Trantirkova;Silvie Foldynova-TrantirkovaCentral European Institute of Technology, Masaryk UniversityBrnoCzech Republiclukas.trantirek@ceitec.muni.czInstitute of Biophysics, v.v.i., ASCRBrnoCzech RepublicSearch for other works by this author on:Lukas TrantirekLukas TrantirekCentral European Institute of Technology, Masaryk UniversityBrnoCzech Republiclukas.trantirek@ceitec.muni.czSearch for other works by this author on:
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