Chapter 16: In-cell NMR Spectroscopy of Nucleic Acids
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Published:09 Dec 2019
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Special Collection: 2019 ebook collectionSeries: New Developments in NMR
S. Dzatko, R. Fiala, R. Hänsel-Hertsch, S. Foldynova-Trantirkova, and L. Trantirek, in In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology, ed. Y. Ito, V. Dötsch, and M. Shirakawa, The Royal Society of Chemistry, 2019, ch. 16, pp. 272-297.
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The structure, dynamics, and interactions of nucleic acids, in particular DNAs, sensitively respond to a broad range of environmental factors, many of them being natively present in the intracellular space. In-cell NMR is a tailored adaptation of conventional high-resolution nuclear magnetic resonance spectroscopy that permits characterization of stereochemical features of nucleic acids directly in living cells. Here we review recent advances and applications of in-cell NMR to nucleic acids. The limitations of this method as well as the general considerations, comprising of critical factors to be accounted for during the planning of in-cell NMR experiments, as well as during the interpretation of in-cell NMR data, are discussed.