In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology
Chapter 15: Primary Drug Screening by In-cell NMR Spectroscopy
Published:09 Dec 2019
S. Dansereau, D. S. Burz, and A. Shekhtman, in In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology, ed. Y. Ito, V. Dötsch, and M. Shirakawa, The Royal Society of Chemistry, 2019, ch. 15, pp. 249-271.
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The successful application of in-cell NMR spectroscopy for primary drug high-throughput screening (HTS), by direct and indirect target engagement in prokaryotes is reviewed. We discuss approaches for identifying drug-binding targets, mapping the target interaction surface and methodologies to rapidly screen small-molecule libraries, as well as nascent strategies for primary drug HTS in eukaryotic cells, including novel methods of target and drug delivery to cells.