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New Developments in NMR
In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology
Edited by
Yutaka Ito;
Yutaka Ito
Tokyo Metropolitan University, Japan
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Masahiro Shirakawa
Masahiro Shirakawa
Kyoto University, Japan
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Hardback ISBN:
978-1-78801-217-1
PDF ISBN:
978-1-78801-307-9
EPUB ISBN:
978-1-83916-093-6
Special Collection:
2019 ebook collection
Series:
New Developments in NMR
No. of Pages:
305
Published online:
09 Dec 2019
Published in print:
13 Dec 2019
Book Chapter
Chapter 2: Protein NMR in Escherichia coli
By
Yansheng Ye
;
Yansheng Ye
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences
Wuhan
Hubei 430071
China
[email protected]
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Conggang Li
Conggang Li
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences
Wuhan
Hubei 430071
China
[email protected]
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Published:09 Dec 2019
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Special Collection: 2019 ebook collectionSeries: New Developments in NMR
Page range:
11 - 26
Citation
Y. Ye and C. Li, in In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology, ed. Y. Ito, V. Dötsch, and M. Shirakawa, The Royal Society of Chemistry, 2019, ch. 2, pp. 11-26.
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During the last two decades NMR methods have been rapidly developed to study proteins in living Escherichia coli cells. This chapter reviews the labeling strategies and sample preparations for protein NMR in E. coli and summarizes some of the key advances including the determination of protein high-resolution 3D structures and the characterization of protein interactions and stabilities in E. coli cells. In addition, the limitations and future applications are discussed.
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