Chapter 2: Fundamentals of Biological Nanopore Electrochemistry
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Published:26 Oct 2020
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Special Collection: 2020 ebook collectionSeries: Detection Science Series
Y. Ying, J. Wang, X. Wu, and Y. Long, in Confining Electrochemistry to Nanopores, ed. Y. Long, The Royal Society of Chemistry, 2020, ch. 2, pp. 9-43.
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In this chapter, we focus on the fundamental techniques and knowledge of biological nanopore electrochemistry. First, we introduce the design and assembly of a nanopore-based single biomolecule interface; these biomolecules are adapted from nature and perfectly confine the single analyte molecule into its lumen volume. Thanks to modern biochemical engineering, each amino acid that composes the nanopore can be fine-tuned for a specific sensing purpose. Finally, as a biomimicking system, an artificial cellular membrane is constructed for nanopore sensing with high robustness as well as less fluidity.