Chapter 2: Fundamentals of Biological Nanopore Electrochemistry
Published:26 Oct 2020
In this chapter, we focus on the fundamental techniques and knowledge of biological nanopore electrochemistry. First, we introduce the design and assembly of a nanopore-based single biomolecule interface; these biomolecules are adapted from nature and perfectly confine the single analyte molecule into its lumen volume. Thanks to modern biochemical engineering, each amino acid that composes the nanopore can be fine-tuned for a specific sensing purpose. Finally, as a biomimicking system, an artificial cellular membrane is constructed for nanopore sensing with high robustness as well as less fluidity.