This chapter reviews important and recent research on mechanically robust amphiphilic polymer co-networks based on α,ω-diacrylated Pluronic F127 [(ethylene glycol)₉₉-b-(propylene glycol)₆₅-b-(ethylene glycol)₉₉] macro-crosslinker copolymerised with acrylamide. Due to the presence of the Pluronic macro-crosslinker, these co-networks are thermo-responsive. Moreover, they can also acquire salt- and pH-responsiveness via the incorporation of a cationic monomer, or both a cationic and an anionic monomer. Co-networks of the latter type, equilibrated in salt solution, could be actuated by an electric field, presenting a fast response and proving themselves as potential materials for use in sensors, actuators, switches and artificial muscles.