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Chromatographic analysis of biogenic amines (BAs) from food samples remains challenging for analytical chemists. Before typical chromatographic analysis of BAs, extraction, pre-concentration, sample clean up, and then the derivatized extract are required as mandatory protocols to improve the sensitivity of the analysis. Since most BAs are non-volatile, the liquid chromatography approach is the obvious choice. Other methods have also been reported, these methods include capillary electrophoresis (CE), ion chromatography (IC), thin-layer chromatography (TLC), and gas chromatography (GC). In this chapter, more emphasis is given for the various chromatographic instrumental conditions, detectors, and columns used for the analysis of BAs from food samples.

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