Inhibitors of Protein–Protein Interactions
Chapter 6: Genetically Encoded SICLOPPS Libraries for the Identification of PPI Inhibitors
Published:25 Nov 2020
M. M. Fernholz, L. M. Windeln, M. Papayova, and A. Tavassoli, in Inhibitors of Protein–Protein Interactions, ed. A. Tavassoli, The Royal Society of Chemistry, 2020, ch. 6, pp. 218-231.
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Genetically encoded cyclic peptide libraries have caused a step-change in hit discovery against some of the most challenging disease targets. Split-intein circular ligation of peptides and proteins (SICLOPPS) enables intracellular generation of cyclic peptide libraries in prokaryotes and eukaryotes. Library production in cells allows functional, cell-based screening for inhibitor identification. Here, we review the use of SICLOPPS screening for the identification of protein–protein interaction inhibitors against a variety of targets.