Detection Methods in Precision Medicine
CHAPTER 3: Proteomics in Precision Medicine
Published:10 Dec 2020
Special Collection: 2020 ebook collectionSeries: Detection Science
Jiangnan Zheng, An He, Yiheng Mao, Lijun Yang, Chris Soon Heng Tan, Ruijun Tian, 2020. "Proteomics in Precision Medicine", Detection Methods in Precision Medicine, Mengsu (Michael) Yang, Michael Thompson
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Systematic profiling of signaling pathways on the proteome level can reveal functionally important proteins that can be used in precision diagnostics, prediction of treatment response and development of personalized therapies. Mass spectrometry (MS)-based proteomics has become the method of choice in this area, given the ability to study thousands of proteins simultaneously. This chapter covers the basics and current state of bottom-up proteomics workflow, including sample preparation, liquid chromatography separations, MS instrumentation, data acquisition strategies, quantification techniques, and data analysis. Moreover, we describe the recent applications of proteomics in the precision medicine field.