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MASSyPup64 ( is a ready-to-use collection of programs and example data, built on a Linux live system, for the analysis of mass spectrometry data and data mining.1  The initial purpose of building a Linux live system was to provide a common informatics set-up for MS data analysis courses.2  However, the system can be run directly from a USB drive and can also be used to process MS data on local drives.

The first version of was based on 32 bit Puppy Linux ( and named ‘MASSyPup’. Since most computers, now, support the 64 bit architecture, the latter versions of MASSyPup, namely ‘MASSyPup64’ are based on the 64 bit live Linux operating systems, such as Fatdog64 ( and antiX (

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