DNA Damage, DNA Repair and Disease: Volume 2
Chapter 20: Fidelity Mechanisms of DNA Polymerase Beta
Published:11 Nov 2020
Special Collection: 2020 ebook collectionSeries: Chemical Biology
J. B. Sweasy, in DNA Damage, DNA Repair and Disease: Volume 2, ed. M. Dizdaroglu, R. S. Lloyd, M. Dizdaroglu, and R. S. LLoyd, The Royal Society of Chemistry, 2020, ch. 20, pp. 104-128.
Download citation file:
DNA polymerase β functions in DNA repair and has been extensively characterized as a model enzyme in order to ascertain the molecular mechanisms of DNA polymerase catalysis and fidelity. Research on the catalytic mechanism of polymerase β with a particular emphasis on substrate specificity is reviewed here. Notably, phosphodiester bond formation is the rate-limiting step of the reaction. Recent work has also provided strong evidence for an induced fit mechanism underlying correct selection of the nucleotide substrate for incorporation into DNA.