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This chapter summarizes the various types of functionalized macromolecules used as electrode modifiers for electrochemical sensor applications. The functionalized macromolecules serve as recognition and/or transducer elements on the disposable electrode system. The electrocatalytic mechanism during the electroanalysis was evaluated for the developed disposable sensor systems. The sensor systems were used for environmental analysis and detection of various diseases as well as viral and bacterial infections. Real-time samples will be explained in detail for the fabricated disposable sensors. Also, the merits and demerits of these functionalized macromolecules-based biosensors are highlighted to provide room for further developments.

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