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Nanopore signal processing generally consists of baseline removal, denoising, spike detection, extraction of spikes' amplitude and duration, and classification of spikes. Here we present an improved method for baseline removing, an optimized algorithm for denoising the nanopore signals, a novel method for spike detection that detects all the translocation spikes more correctly, and an improved and physically meaningful algorithm for measuring the duration and amplitude of the translocation spikes.

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