Glycerophospholipids are phosphodiesters of glycerol and an alcohol referred to as the polar headgroup. The two glycerol carbinol groups are typically esterified with long chain fatty acyl groups which impart hydrophobic character to these otherwise polar molecules. The simplest glycerophospholipid is phosphatidic acid which is a monophosphoester of diacylglycerol. The common polar headgroups are choline, ethanolamine, serine, inositol, and glycerol which divide the phospholipids into different classes. This chapter discusses glycerophosphocholine lipids (PC), glycerophosphoethanolamine lipids (PE), phosphatidylserine (PS), phosphatidic acid (PA), phosphatidylinositol (PI), phosphatidylglycerol (PG), bis(acyl-lysophosphatidyl)glycerol (BMP) and cardiolipin.